| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
06/12/2021 |
|
Data da última atualização: |
06/12/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
GIGLIOTI, R.; OKINO, C. H.; AZEVEDO, B. T.; GUTMANIS, G.; KATIKI, L. M.; OLIVEIRA, M. C. de S.; VERCESI FILHO, A. E. |
|
Afiliação: |
RODRIGO GIGLIOTI, IZ; CINTIA HIROMI OKINO, CPPSE; BIANCA TAINA AZEVEDO, IZ; GUNTA GUTMANIS, IZ; LUCIANA MORITA KATIKI, IZ; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; ANIBAL EUGENIO VERCESI FILHO, IZ. |
|
Título: |
Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Food Chemistry: Molecular Sciences, v.3, 2021, 100055. |
|
Páginas: |
6 p. |
|
DOI: |
https://doi.org/10.1016/j.fochms.2021.100055 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products. |
|
Palavras-Chave: |
B Casein; LNA probe; Real time PCR; Sensitivity. |
|
Thesaurus Nal: |
Alleles. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228550/1/NovelLNAProbe.pdf
|
|
Marc: |
LEADER 01730naa a2200277 a 4500
001 2137153
005 2021-12-06
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.fochms.2021.100055$2DOI
100 1 $aGIGLIOTI, R.
245 $aNovel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples.$h[electronic resource]
260 $c2021
300 $a6 p.
520 $aThe rising consumption of A2 milk and its derivatives in recent years has garnered attention from both consumers and producers, mainly due its possible health benefits, such as enhanced digestion and easier absorption. Thus, a novel real-time PCR using a combination of locked nucleic acid modified (LNA) conjugated probes was developed to genotype A1 and A2 alleles of B-casein gene (CSN2) and to detect and quantify the A1 presence in A2 samples. The limit of detection for each probe (A1 and A2) was evaluated using decreasing serial dilutions. Besides, the sensitivity of A1 allele detection in the A2 samples was also tested. The limits of detection of A1 and A2 alleles were 6 copies, while for A1 allele detection in A2 samples was 7.5 copies (1%). The LNA-probe based method was found to be rapid, robust, highly sensitive, cost effective, and can be employed as screening test to certificate the A2 dairy products.
650 $aAlleles
653 $aB Casein
653 $aLNA probe
653 $aReal time PCR
653 $aSensitivity
700 1 $aOKINO, C. H.
700 1 $aAZEVEDO, B. T.
700 1 $aGUTMANIS, G.
700 1 $aKATIKI, L. M.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aVERCESI FILHO, A. E.
773 $tFood Chemistry: Molecular Sciences$gv.3, 2021, 100055.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 5 | |
| 2. |  | GIGLIOTI, R.; OKINO, C. H.; AZEVEDO, B. T.; GUTMANIS, G.; KATIKI, L. M.; OLIVEIRA, M. C. de S.; VERCESI FILHO, A. E. Novel LNA probe-based assay for the A1 and A2 identification ofB-casein gene in milk samples. Food Chemistry: Molecular Sciences, v.3, 2021, 100055. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|    |
| 3. | | AZEVEDO, B. T.; VERCESI FILHO, A. E.; GUTMANIS, G.; VERISSIMO, C. J.; KATIKI, L. M.; OKINO, C. H.; OLIVEIRA, M. C. de S.; GIGLIOTI, R. New sensitive methods for fraud detection in buffalo dairy products. International Dairy Journal, v.117, 105013, jun. 2021. 9 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 4. | | GIGLIOTI, R.; OKINO, C. H.; AZEVEDO, B. T.; WEDY, B. C. R.; GUTMANIS, G.; VERÍSSIMO, C. J.; KATIKI, L. M.; VERCESI FILHO, A. E.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S. Semi-quantitative evaluation of Babesia bovis and B. bigemina infection levels estimated by HRM analysis. Ticks and Tick-borne Diseases, v.12, n.5, 101753, sep. 2021. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 5. | | GIGLIOTI, R.; OLIVEIRA, H. N. de; GUTMANIS, G.; LUCIANI, G. F.; AZEVEDO, B. T.; FIORIN, C. F. de C.; ANDRADE, M. F. de; SILVA, M. A. F.; VERCESI FILHO, A. E.; KATIKI, L. M.; OKINO, C. H.; OLIVEIRA, M. C. de S.; VERÍSSIMO, C. J. Correlations and repeatability between Babesia spp. infection levels using two dairy cattle breeding systems. Experimental and Applied Acarology, v. 81, p. 599-607, jul. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| Registros recuperados : 5 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
